Henry Slade, the 21 year-old Exeter Chiefs flyhalf will be vice-captain.
RFU Head of International Player Development Joe Lydon said: “This England squad is full of talent and will make for an entertaining match against a strong Barbarians side with over 700 caps between them.
“We see this match as an important development opportunity for the players and many of them gained valuable experience working with the senior squad and coaches over the last week before they departed for New Zealand.
“We have seen 10 players involved in these Barbarians fixtures over the last two years who have gone on to get caps for England.
“The team are really looking forward to running out and playing in front of the home crowd at Twickenham which has had a fantastic atmosphere this season,” he added.
England XV: 15 Elliot Daly, 14 Semesa Rokoduguni, 13 Jonathan Joseph, 12 Sam Hill, 11 Charlie Sharples, 10 Henry Slade, 9 Joe Simpson, 8 Dave Ewers, 7 Luke Wallace, 6 Jamie Gibson, 5 Graham Kitchener, 4 Michael Paterson, 3 Kyle Sinckler, 2 Rob Buchanan, 1 Ross Harrison.
Replacements: 16 Tommy Taylor, 17 Fraser Balmain, 18 Will Collier, 19 Charlie Matthews, 20 James Gaskell, 21 Dan Robson, 22 Ollie Devoto, 23 Rob Miller.
Date: Sunday, June 1
Venue: Twickenham, London
Kick-off: 15.00 (14.00 GMT)
Referee: Mathieu Raynal (France)
Assistant referees: tba (Wales)
TMO: Gareth Simmonds (Wales)